Abstract

 Toxoplasma gondii infections are prevalent in humans and animals. This study aimed to detection SAG2 gene by nested PCR method in Iraqi thalassemic patients infected with T. gondii. Patients sample consist of 165 were collected from Al- Karma Teaching Hospital in Baghdad, Iraq, also 80 case of healthy individuals their age ranges from 2-45 years, with mean (15.387±0.627) during the period from March to June 2022 cases Iraq. Anti-Toxoplasma IgM/IgG antibodies were using in detection toxoplasmosis by Chemiluminescent microparticles immunoassay (CMIA). Results revealed that there is a significant difference P≤0.001 in the level of Toxoplasma IgG antibody between group of negative control group (0.5616±0.246 IU/ml) with β–thalassemic major patients infected with toxoplasmosis (24.985±6.101IU/ml) and positive control infected with toxoplasmosis (35.59±8.336 IU/ml), also the difference was significant (P<0.001) between the group of positive control infected with toxoplasmosis (35.59±8.336 IU/ml) with the group of with β–thalassemic major patients (0.489±0.084 IU/ml), however all groups were seronegative for anti- Toxoplasma IgM antibody.  Molecular detection was done by nested PCR method to detect SAG2 gene in studied samples. Results showed that 6/25(24.0%) of β-thalassemic major patients infected with toxoplasmosis have positive response SAG2 gene by nested PCR assay, while others groups were negative for SAG2 in the same assay. In conclusion, the Iraqi B-thalassemia patients of the current study were infected with chronic toxoplasmosis. Furthermore, result showed present of T. gondii SAG2 gene were detected in the blood samples of B-thalassemia patients with toxoplasmosis and positive control