Document Type : Research Paper
Abstract
Serratia liquifaciences, which have the potential to create butanol after degrading cellulose components in animal waste in the presence of Oxegen at 37 ºC, were isolated from animal samples wastes and cultivated on nutritive broth with various concentrations of butanol.
After that, the experiments were carried out using particular media containing various dilutions of animal feces injected with isolated 1 mg/l bacteria Serratia liquifaciences after adjusted pH to 7.0, then results refer to exist high rate of butanol, reaching to 164.6g/l, after incubation up to 10 days, at 37ºC.
Results showed that optical density (OD) for culture after different period of incubation time, were recorded at 540 nm with (0.004, 0.0066, 0.013, 0.014, 0.39 and 0.66), also, using another sample as in paper wastes to test the ability of bacteria to produce butanol. So, the obtained concentrations of glucose were (19, 18.3, 22, 23, 22 and 24) mg /l, respectively, and the obtained butanol by this methods19.7g/l.
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