Abstract

One of the most important modern approaches to preventing antibiotic resistance is the production of antibiotics utilizing nanotechnology. To make nano-antibiotics, Fe2O3 particles were generated using the chemical precipitation approach and combined with Tetracycline using the physical method, utilizing ultrasonic equipment. The TCS nanoparticle dispersion in the tetracycline particle-matrix was found to be stable. The resultant nanocomposites were evaluated using XRD, EDX, and SEM techniques, and their properties were compared to those in the ICDD database [ICDD card no. 0664-33]. The average crystal size of Fe₂O₃ was 36.75 nm, whereas the average crystal size of Fe₂O₃+TCS was 28.10 nm. TCS bound with Fe₂O₃ was tested for bacterial activity. It was compared to normal tetracycline particles by evaluating the lowest inhibitory concentration of two species of bacteria using an ELISA method. Fe₂O₃+TCS NPs had a MIC of 32 µg/ml against Staphylococcus, indicating good bactericidal effectiveness. In comparison, the MIC of standard TCs was 512 µg/ml, showing that the aforesaid chemical was more effective than regular TCs. In comparison to conventional TCs of 512 µg/ml, Fe₂O₃+TCS NPs showed a MIC of 128 µg/ml against Pseudomonas aeruginosa. Hemolysis was also performed to test the compound's toxicity (Fe₂O₃+TCS NPs). The compounds were found to be safe at all concentrations except 500 µg/ml, according to the results of the experiments.