Document Type : Research Paper
Abstract
This paper describes a method for determining uric acid in urine electrochemically through its reaction with uricase (UOx). This procedure is based on the changes in electrical potential associated with the enzymatic reaction of the working solution with uric acid as the reaction occurs, depending on the amount of decrease in the wavelength of the working solution with the addition of uric acid.
When electrochemical measurements of the study sample were performed in phosphate buffer solution (pH 7.0), the appearance of three reduction peaks was observed. The first wave, which is the main reduction wave, was adopted at a voltage of (-0.32) volts. These values were measured on the reference electrode (calomel reference) after applying a voltage ranging between (-0.8-0) volts. As for the rest of the waves, they were secondary and insensitive, so they were not adopted.
The results of electrochemical methods were compared with those obtained by conventional methods of measuring uric acid using spectrophotometry. No systematic errors were observed, but the results were largely identical, taking into account that electrochemical methods gave better results compared with the spectroscopic method is economical, accurate and fast
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